Relative Quantification of Na V 1.1 Protein in Mouse Brains Using a Meso Scale Discovery-Electrochemiluminescence (MSD-ECL) Method

Relative Quantification of Na V 1.1 Protein in Mouse Brains Using a Meso Scale Discovery-Electrochemiluminescence (MSD-ECL) Method

Densitometric evaluation is commonly used to quantify Na<sub>V</sub>1.1 protein on immunoblots, though the sensitivity and dilution linearity of the strategy are normally poor. Right here we current a <em>protocol</em> for quantification of Na<sub>V</sub>1.1 in mouse mind tissues utilizing a Meso Scale Discovery-Electrochemiluminescence (<em>MSD</em>-ECL) technique. <em>MSD</em>-ECL is predicated on ELISA (enzyme-linked immunosorbent assay) and makes use … Read more